Real-time polymerase chain reaction (PCR) chemistry is a technique frequently used by biological research labs worldwide for replicating and quantifying DNA. The process could be optimized by eliminating air bubbles commonly present in the reaction. The Clinic team has worked with Applied Biosystems to develop a next-generation microfluidics concept which addresses this task. The design utilizes a collapsed membrane which expands under pressure to form airless reaction sites as the device is filled with liquid reagents.