Genentech employs sterile lab scale bioreactors to engineer drug-based medical solutions. Typically, bioreactors are manually sampled to monitor their internal conditions and to ensure cell culture integrity. An automated sampling system was introduced to increase sampling efficiency and to reduce ergonomic risk. However, foreign contaminants compromised the sterility of the system. The Genentech Clinic Team has designed, built, and tested two distinct sterile barriers that will increase the reliability of the pre-existing automated sampling system.