Nanofibers, fibers 100 to 1,000 times narrower than a human hair, have a wide range of technological applications because of their outstanding surface area to mass ratio. Applications include medical filtration and advanced lithium-ion batteries. An infrastructure does not yet exist for the storage and transport of nanofibers for use at secondary processing facilities. To this end, we designed, built, and tested a system to store nanofibers on a spool and unwind them for reuse later.