In order to improve the accuracy and effectiveness of live animal in vivo fluorescent imaging, UVP tasked a team of Harvey Mudd College and Keck Graduate Institute students to design, simulate, and test innovative imaging systems to achieve unparalleled illumination uniformity over five degrees of freedom. Uniform lighting is needed for quantitative analysis of in vivo images, which are used for cancer and disease research. Additionally, the team developed novel methodologies for measuring lighting uniformity.