Imaging and sequencing individual cells

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Speaker(s): Aaron Streets

Quantitative cellular imaging with coherent Raman microscopy reveals morphological characteristics and chemical composition at the single-cell level. Meanwhile recent advances in high-throughput sequencing have enabled whole-transcriptome profiling of gene expression in single cells. Both measurements can uncover heterogeneity in cellular populations that would otherwise be obscured in ensemble measurement. Furthermore both imaging and gene expression profiling can be used to quantify cell state during differentiation. However, in order to infer the relationship between gene expression and morphological phenotypes, it is necessary to image and sequence the same single cell. We use a microfluidic platform to couple imaging and RNA sequencing of single cells and present recent developments on how to analyze large, multimodal, single-cell datasets.

Colloquium

Junior and senior physics majors attend our biweekly colloquium series, held on Tuesday afternoons at 4:30 p.m. in Shanahan B460. The talks are open to all students and to the public, and are frequently attended by scientists from the other Claremont Colleges, Cal Poly Pomona, and others. The series features speakers from a broad range of institutions and fields of physics.